Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition
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elife-33150-v1.pdf
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Author(s)
Hara, Masatoshi
Lourido, Sebastian
Petrova, Boryana
Lou, Hua Jane
Von Stetina, Jessica R
Kashevsky, Helena
Turk, Benjamin E
Orr-Weaver, Terry
Date Issued
February 2018
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Hara, Masatoshi, et al. “Identification of PNG Kinase Substrates Uncovers Interactions with the Translational Repressor TRAL in the Oocyte-to-Embryo Transition.” eLife 7 (February 2018): e33150 © Hara et al
Version
Final published version
Abstract
The Drosophila Pan Gu (PNG) kinase complex regulates hundreds of maternal mRNAs that become translationally repressed or activated as the oocyte transitions to an embryo. In a previous paper (Hara et al., 2017), we demonstrated PNG activity is under tight developmental control and restricted to this transition. Here, examination of PNG specificity showed it to be a Thrkinase yet lacking a clear phosphorylation site consensus sequence. An unbiased biochemical screen for PNG substrates identified the conserved translational repressor Trailer Hitch (TRAL). Phosphomimetic mutation of the PNG phospho-sites in TRAL reduced its ability to inhibit translation in vitro. In vivo, mutation of tral dominantly suppressed png mutants and restored Cyclin B protein levels. The repressor Pumilio (PUM) has the same relationship with PNG, and we also show that PUM is a PNG substrate. Furthermore, PNG can phosphorylate BICC and ME31B, repressors that bind TRAL in cytoplasmic RNPs. Therefore, PNG likely promotes translation at the oocyte-to-embryo transition by phosphorylating and inactivating translational repressors.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Attribution 4.0 International (CC BY 4.0)
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DOI of Published Version
https://doi.org/10.7554/eLife.33150
