Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers
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Bhatia_Disease detection.pdf
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2.52 MB
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Author(s)
Gaylord, Shonda T.
Ngan, Kevin C.
Dumont Milutinovic, Milena
Kwong, Gabriel A.
Bhatia, Sangeeta N.
Walt, David R.
Warren, Andrew David
Date Issued
September 2014
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Citation
Warren, Andrew D., Shonda T. Gaylord, Kevin C. Ngan, Milena Dumont Milutinovic, Gabriel A. Kwong, Sangeeta N. Bhatia, and David R. Walt. “Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers.” Journal of the American Chemical Society 136, no. 39 (October 2014): 13709–13714. © 2014 American Chemical Society
Version
Final published version
Abstract
The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for “microdosing.”
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ja505676h
