Suspended microchannel resonators for ultralow volume universal detection
Name
302264229-MIT.pdf
Description
Full printable version
Size
7.32 MB
Format
Adobe PDF
Checksum (MD5)
6d7cfcd08fd4ea3a76b4697b7783da9a
Author(s)
Son, Sungmin
Advisor(s)
Scott Manalis.
Date Issued
2008
Publisher
Massachusetts Institute of Technology
Abstract
Universal detectors that maintain high sensitivity as the detection volume is reduced to the sub-nanoliter scale can enhance the utility of miniaturized total analysis systems ([mu]-TAS). Here the unique scaling properties of the suspended microchannel resonator (SMR) are exploited to show universal detection in a 10 pL analysis volume with a density detection limit of ~1 ([mu]g/cm³ (10 Hz bandwidth) and a linear dynamic range of six decades. Analytes with low UV extinction coefficients such as polyethylene glycol (PEG) 8 KDa, glucose, and glycine are measured with molar detection limits of 0.66 ([mu]M, 13.5 ([mu]M, and 31.6 ([mu]M, respectively. To demonstrate the potential for real-time monitoring, gel filtration chromatography was used to separate different molecular weights of PEG as the SMR acquired a chromatogram by measuring the eluate density. This work suggests that the SMR could offer a simple and sensitive universal detector for various separation systems from liquid chromatography to capillary electrophoresis. Moreover, since the SMR is itself a microfluidic channel, it can be directly integrated into ([mu]-TAS without compromising overall performance.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2008.
Includes bibliographical references (leaves 32-33).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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