An N-stage cascade of phosphorylation cycles as an insulation device for synthetic biological circuits
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RushinaECC2016.pdf
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Author(s)
Shah, Rushina Jaidip
Del Vecchio, Domitilla
Date Issued
July 2016
Journal
2016 European Control Conference (ECC)
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Citation
Shah, Rushina, and Domitilla Del Vecchio. “An N-Stage Cascade of Phosphorylation Cycles as an Insulation Device for Synthetic Biological Circuits.” 2016 European Control Conference (ECC) (June 2016), Aalborg, Denmark, Institute of Electrical and Electronics Engineers (IEEE), 2016. © 2016 EUCA
Version
Author's final manuscript
Abstract
Single phosphorylation cycles have been found to have insulation device abilities, that is, they attenuate the effect of retroactivity applied by downstream systems and hence facilitate modular design in synthetic biology. It was recently discovered that this retroactivity attenuation property comes at the expense of an increased retroactivity to the input of the insulation device, wherein the device slows down the signal it receives from its upstream system. In this paper, we demonstrate that insulation devices built of cascaded phosphorylation cycles can break this tradeoff, allowing to attenuate the retroactivity applied by downstream systems while keeping a small retroactivity to the input. In particular, we show that there is an optimal number of cycles that maximally extends the linear operating region of the insulation device while keeping the desired retroactivity properties, when a common phosphatase is used. These findings provide optimal design strategies of insulation devices for synthetic biology applications.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1109/ECC.2016.7810557
