Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
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Boyden_Simultaneous whole.pdf
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Author(s)
Prevedel, Robert
Yoon, Young-Gyu
Hoffmann, Maximilian
Pak, Nikita
Wetzstein, Gordon
Kato, Saul
Schrödel, Tina
Raskar, Ramesh
Zimmer, Manuel
Boyden, Edward Stuart
Date Issued
May 2014
Journal
Nature Methods
Publisher
Springer Nature
Citation
Prevedel, Robert, Young-Gyu Yoon, Maximilian Hoffmann, Nikita Pak, Gordon Wetzstein, Saul Kato, Tina Schrödel, Ramesh Raskar, Manuel Zimmer, Edward S. Boyden, and Alipasha Vaziri. “Simultaneous Whole-Animal 3D Imaging of Neuronal Activity Using Light-Field Microscopy.” Nature Methods 11, no. 7 (May 18, 2014): 727–730.
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Author's final manuscript
Abstract
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
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DOI of Published Version
https://doi.org/10.1038/nmeth.2964
