A single-cell liver atlas of Plasmodium vivax infection
Name
1-s2.0-S1931312822001640-main.pdf
Description
Published version
Size
4.21 MB
Format
Adobe PDF
Checksum (MD5)
59d60bde63cb60cd10c9d892de801f3c
Author(s)
Mancio-Silva, Liliana
Gural, Nil
Real, Eliana
Wadsworth, Marc H
Butty, Vincent L
March, Sandra
Nerurkar, Niketa
Hughes, Travis K
Roobsoong, Wanlapa
Fleming, Heather E
Date Issued
April 2022
Journal
Cell Host & Microbe
Publisher
Elsevier BV
Citation
Mancio-Silva, Liliana, Gural, Nil, Real, Eliana, Wadsworth, Marc H, Butty, Vincent L et al. 2022. "A single-cell liver atlas of Plasmodium vivax infection." Cell Host & Microbe.
Version
Final published version
Abstract
Malaria-causing Plasmodium vivax parasites can linger in the human liver for weeks to years and reactivate to cause recurrent blood-stage infection. Although they are an important target for malaria eradication, little is known about the molecular features of replicative and non-replicative intracellular liver-stage parasites and their host cell dependence. Here, we leverage a bioengineered human microliver platform to culture patient-derived P. vivax parasites for transcriptional profiling. Coupling enrichment strategies with bulk and single-cell analyses, we capture both parasite and host transcripts in individual hepatocytes throughout the course of infection. We define host- and state-dependent transcriptional signatures and identify unappreciated populations of replicative and non-replicative parasites that share features with sexual transmissive forms. We find that infection suppresses the transcription of key hepatocyte function genes and elicits an anti-parasite innate immune response. Our work provides a foundation for understanding host-parasite interactions and reveals insights into the biology of P. vivax dormancy and transmission.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Chemistry
Ragon Institute of MGH, MIT and Harvard
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.chom.2022.03.034
