Conversion of a yeast prion protein to an infectious form in bacteria
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Garrity-2010-Conversion of a yeas.pdf
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Author(s)
Lindquist, Susan
Dong, Jijun
Garrity, Sean J.
Sivanathan, Viknesh
Hochschild, Ann
Date Issued
May 2010
Journal
Proceedings of the National Academy of Sciences of the United States of America. (PNAS)
Publisher
National Academy of Sciences (U.S.)
Citation
Garrity, S. J. et al. “Conversion of a yeast prion protein to an infectious form in bacteria.” Proceedings of the National Academy of Sciences 107.23 (2010): 10596-10601. Copyright ©2011 by the National Academy of Sciences
Version
Final published version
Abstract
Prions are infectious, self-propagating protein aggregates that have been identified in evolutionarily divergent members of the eukaryotic domain of life. Nevertheless, it is not yet known whether prokaryotes can support the formation of prion aggregates. Here we demonstrate that the yeast prion protein Sup35 can access an infectious conformation in Escherichia coli cells and that formation of this material is greatly stimulated by the presence of a transplanted [PSI+] inducibility factor, a distinct prion that is required for Sup35 to undergo spontaneous conversion to the prion form in yeast. Our results establish that the bacterial cytoplasm can support the formation of infectious prion aggregates, providing a heterologous system in which to study prion biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.0913280107
