A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device
Name
2738.2013.373569.pdf
Size
2.08 MB
Format
Adobe PDF
Checksum (MD5)
859767ae0339c5ac143579eee3a32773
Author(s)
Zhang, Chen
Jang, Sunyoung
Amadi, Ovid C.
Shimizu, Koichi
Lee, Richard T.
Mitchell, Richard Neal
Date Issued
2013
Journal
BioMed Research International
Publisher
Hindawi Publishing Corporation
Citation
Zhang, Chen et al. “A Sensitive Chemotaxis Assay Using a Novel Microfluidic Device.” BioMed Research International 2013 (2013): 1–8.
Version
Final published version
Abstract
Existing chemotaxis assays do not generate stable chemotactic gradients and thus—over time—functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We describe here a novel microfluidic device for sensitive assay of cellular migration and show its application for evaluating the chemotaxis of smooth muscle cells in a chemokine gradient.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1155/2013/373569
