Activation of GATA binding protein 6 (GATA6) sustains oncogenic lineage-survival in esophageal adenocarcinoma
Name
Lin-2012-Activation of GATA b.pdf
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Author(s)
Lin, Lin
Meyerson, Matthew L.
Lockwood, William W.
Wang, Zhuwen
Silvers, Amy L.
Bass, Adam J.
Thomas, Dafydd G.
Chang, Andrew C.
Lin, Jules
Orringer, Mark B.
Date Issued
March 2012
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Citation
Lin, L. et al. “Activation of GATA Binding Protein 6 (GATA6) Sustains Oncogenic Lineage-survival in Esophageal Adenocarcinoma.” Proceedings of the National Academy of Sciences (2012). ©2012 by the National Academy of Sciences
Version
Final published version
Abstract
Gene amplification is a tumor-specific event during malignant transformation. Recent studies have proposed a lineage-dependency (addiction) model of human cancer whereby amplification of certain lineage transcription factors predisposes a survival mechanism in tumor cells. These tumor cells are derived from tissues where the lineage factors play essential developmental and maintenance roles. Here, we show that recurrent amplification at 18q11.2 occurs in 21% of esophageal adenocarcinomas (EAC). Utilization of an integrative genomic strategy reveals a single gene, the embryonic endoderm transcription factor GATA6, as the selected target of the amplification. Overexpression of GATA6 is found in EACs that contain gene amplification. We find that EAC patients whose tumors carry GATA6 amplification have a poorer survival. We show that ectopic expression of GATA6, together with FGFR2 isoform IIIb, increases anchorage-independent growth in immortalized Barrett's esophageal cells. Conversely, siRNA-mediated silencing of GATA6 significantly reduces both cell proliferation and anchorage-independent growth in EAC cells. We further demonstrate that induction of apoptotic/anoikis pathways is triggered upon silencing of GATA6 in EAC cells but not in esophageal squamous cells. We show that activation of p38α signaling and up-regulation of TNF-related apoptosis-inducing ligand are detected in apoptotic EAC cells upon GATA6 deprivation. We conclude that selective gene amplification of GATA6 during EAC development sustains oncogenic lineage-survival of esophageal adenocarcinoma.
MIT Department
Broad Institute of MIT and Harvard
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DOI of Published Version
https://doi.org/10.1073/pnas.1011989109
