Single-shot dual-wavelength interferometric microscopy
Name
So_Single-shot dual-wavelength interferometric microscopy.pdf
Description
Accepted version
Size
1.26 MB
Format
Adobe PDF
Checksum (MD5)
fd4799124a510d978f570e9398c9e49b
Author(s)
Hosseini, Poorya
Yaqoob, Zahid
So, Peter T.C.
Jin, Di,Ph.D.Massachusetts Institute of Technology.
Date Issued
March 2018
Journal
Methods
Publisher
Elsevier BV
Citation
Hosseini, Poorya, et al. “Single-Shot Dual-Wavelength Interferometric Microscopy.” Methods 136 (March 2018): pp. 35–39.
Version
Author's final manuscript
Abstract
Interferometric microscopy (IM) can provide complex field information of the biological samples with high spatial and temporal resolution with virtually no photodamage. Measuring wavelength-dependent information in particular has a wide range of applications from cell and tissue refractometry to the cellular biophysical measurements. IM measurements at multiple wavelengths are typically associated with a loss in temporal resolution, field of view, stability, sensitivity, and may involve using expensive equipment such as tunable filters or spatial light modulators. Here, we present a novel and simple design for an interferometric microscope that provides single-shot off-axis interferometric measurements at two wavelengths by encoding the two spectral images at two orthogonal spatial frequencies that allows clean separation of information in the Fourier space with no resolution loss. We demonstrated accurate simultaneous quantification of polystyrene bead refractive indices at two wavelengths.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.ymeth.2017.10.006
