Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration
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Reddien_Transcriptome analysis.pdf
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Author(s)
Lapan, Sylvain W.
Reddien, Peter
Lapan, Sylvain W.
Date Issued
August 2012
Journal
Cell Reports
Publisher
Elsevier B.V.
Citation
Lapan, Sylvain W., and Peter W. Reddien. “Transcriptome Analysis of the Planarian Eye Identifies Ovo as a Specific Regulator of Eye Regeneration.” Cell Reports 2, no. 2 (August 2012): 294–307.
Version
Final published version
Abstract
Among the millions of invertebrate species with visual systems, the genetic basis of eye development and function is well understood only in Drosophila melanogaster. We describe an eye transcriptome for the planarian Schmidtea mediterranea. Planarian photoreceptors expressed orthologs of genes required for phototransduction and microvillus structure in Drosophila and vertebrates, and optic pigment cells expressed solute transporters and melanin synthesis enzymes similar to those active in the vertebrate retinal pigment epithelium. Orthologs of several planarian eye genes, such as bestrophin-1 and Usher syndrome genes, cause eye defects in mammals when perturbed and were not previously described to have roles in invertebrate eyes. Five previously undescribed planarian eye transcription factors were required for normal eye formation during head regeneration. In particular, a conserved, transcription-factor-encoding ovo gene was expressed from the earliest stages of eye regeneration and was required for regeneration of all cell types of the eye.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2012.06.018
