Vaccine targeting to mucosal lymphoid tissues promotes humoral immunity in the gastrointestinal tract
Name
sciadv.adn7786.pdf
Description
Published version
Size
3.36 MB
Format
Adobe PDF
Checksum (MD5)
7673ca308a2835333f0e685e1292f121
Author(s)
Kocabiyik, Ozgun
Amlashi, Parastoo
Vo, A Lina
Suh, Heikyung
Rodriguez-Aponte, Sergio A
Dalvie, Neil C
Love, J Christopher
Andrabi, Raiees
Irvine, Darrell J
Date Issued
May 29, 2024
Journal
Science Advances
Publisher
American Association for the Advancement of Science
Citation
Ozgun Kocabiyik et al. ,Vaccine targeting to mucosal lymphoid tissues promotes humoral immunity in the gastrointestinal tract.Sci. Adv. 10, eadn7786 (2024).
Version
Final published version
Abstract
Viruses, bacteria, and parasites frequently cause infections in the gastrointestinal tract, but traditional vaccination strategies typically elicit little or no mucosal antibody responses. Here, we report a strategy to effectively concentrate immunogens and adjuvants in gut-draining lymph nodes (LNs) to induce gut-associated mucosal immunity. We prepared nanoemulsions (NEs) based on biodegradable oils commonly used as vaccine adjuvants, which encapsulated a potent Toll-like receptor agonist and displayed antigen conjugated to their surface. Following intraperitoneal administration, these NEs accumulated in gut-draining mesenteric LNs, priming strong germinal center responses and promoting B cell class switching to immunoglobulin A (IgA). Optimized NEs elicited 10- to 1000-fold higher antigen-specific IgG and IgA titers in the serum and feces, respectively, compared to free antigen mixed with NE, and strong neutralizing antibody titers against severe acute respiratory syndrome coronavirus 2. Thus, robust gut humoral immunity can be elicited by exploiting the unique lymphatic collection pathways of the gut with a lymph-targeting vaccine formulation.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Ragon Institute of MGH, MIT and Harvard
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1126/sciadv.adn7786
