Xylan Structure and Dynamics in Native Brachypodium Grass Cell Walls Investigated by Solid-State NMR Spectroscopy
Name
acsomega.1c01978.pdf
Description
Published version
Size
7.62 MB
Format
Adobe PDF
Checksum (MD5)
0fc6d26cce3dec164532afdd23ea5a06
Author(s)
Duan, Pu
Kaser, Samuel J
Lyczakowski, Jan J
Phyo, Pyae
Tryfona, Theodora
Dupree, Paul
Hong, Mei
Date Issued
2021
Journal
ACS Omega
Publisher
American Chemical Society (ACS)
Citation
Duan, Pu, Kaser, Samuel J, Lyczakowski, Jan J, Phyo, Pyae, Tryfona, Theodora et al. 2021. "Xylan Structure and Dynamics in Native Brachypodium Grass Cell Walls Investigated by Solid-State NMR Spectroscopy." ACS Omega, 6 (23).
Version
Final published version
Abstract
The polysaccharide composition and dynamics of the intact stem and leaf cell walls of the model grass Brachypodium distachyon are investigated to understand how developmental stage affects the polysaccharide structure of grass cell walls. 13C enrichment of the entire plant allowed detailed analysis of the xylan structure, side-chain functionalization, dynamics, and interaction with cellulose using magic-angle-spinning solid-state NMR spectroscopy. Quantitative one-dimensional 13C NMR spectra and two-dimensional 13C-13C correlation spectra indicate that stem and leaf cell walls contain less pectic polysaccharides compared to previously studied seedling primary cell walls. Between the stem and the leaf, the secondary cell wall-rich stem contains more xylan and more cellulose compared to the leaf. Moreover, the xylan chains are about twofold more acetylated and about 60% more ferulated in the stem. These highly acetylated and ferulated xylan chains adopt a twofold conformation more prevalently and interact more extensively with cellulose. These results support the notion that acetylated xylan is found more in the twofold screw conformation, which preferentially binds cellulose. This in turn promotes cellulose-lignin interactions that are essential for the formation of the secondary cell wall.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1021/ACSOMEGA.1C01978
