Two-photon excited photoconversion of cyanine-based dyes
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Kwok-2016-Two-photon.pdf
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Author(s)
Choi, Myunghwan
Bhayana, Brijesh
Zhang, Xueli
Ran, Chongzhao
Kwok, Sheldon J.J
Yun, Seok Hyun (Andy)
Date Issued
March 2016
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Kwok, Sheldon J. J. et al. “Two-Photon Excited Photoconversion of Cyanine-Based Dyes.” Scientific Reports 6 (2016): 23866. © 2016 Macmillan Publishers Limited
Version
Final published version
Abstract
The advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
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DOI of Published Version
https://doi.org/10.1038/srep23866
