Genomic Analysis of Parent-of-Origin Allelic Expression in Arabidopsis thaliana Seeds
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Gehring-2011-Genomic Analysis of.pdf
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Author(s)
Gehring, Mary
Missirian, Victor
Henikoff, Steven
Date Issued
August 2011
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Gehring, Mary, Victor Missirian, and Steven Henikoff. “Genomic Analysis of Parent-of-Origin Allelic Expression in Arabidopsis thaliana Seeds.” Ed. Robert Feil. PLoS ONE 6 (2011): e23687.
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Final published version
Abstract
Differential expression of maternally and paternally inherited alleles of a gene is referred to as gene imprinting, a form of epigenetic gene regulation common to flowering plants and mammals. In plants, imprinting primarily occurs in the endosperm, a seed tissue that supports the embryo during its growth and development. Previously, we demonstrated that widespread DNA demethylation at remnants of transposable elements accompanies endosperm development and that a subset of these methylation changes are associated with gene imprinting. Here we assay imprinted gene expression genome-wide by performing high-throughput sequencing of RNA derived from seeds of reciprocal intraspecific crosses. We identify more than 200 loci that exhibit parent-of-origin effects on gene expression in the endosperm, including a large number of transcription factors, hormone biosynthesis and response genes, and genes that encode regulators of epigenetic information, such as methylcytosine binding proteins, histone methyltransferases, and chromatin remodelers. The majority of these genes are partially, rather than completely, imprinted, suggesting that gene dosage regulation is an important aspect of imprinted gene expression.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0023687
