Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations
Name
fnsyn-12-00006.pdf
Description
Published version
Size
5.84 MB
Format
Adobe PDF
Checksum (MD5)
0a68c2934685ea83a2ce33313377aa52
Author(s)
Lavin, Thomas K.
Jin, Lei
Lea, Nicholas
Wickersham, Ian R.
Date Issued
February 2020
Journal
Frontiers in Synaptic Neuroscience
Publisher
Frontiers Media SA
Citation
Lavin, Thomas K. et al. “Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations.” Frontiers in Synaptic Neuroscience 12 (2020): Article 6 © 2020 The Author(s)
Version
Final published version
Abstract
Monosynaptically-restricted transsynaptic tracing using deletion-mutant rabies virus (RV) has become a widely used technique in neuroscience, allowing identification, imaging, and manipulation of neurons directly presynaptic to a starting neuronal population. Its most common implementation is to use Cre mouse lines in combination with Cre-dependent “helper” adeno-associated viral vectors (AAVs) to supply the required genes to the targeted population before subsequent injection of a first-generation (ΔG) rabies viral vector. Here we show that the efficiency of transsynaptic spread and the degree of nonspecific labeling in wild-type control animals depend strongly on the concentrations of these helper AAVs. Our results suggest practical guidelines for achieving good results.
MIT Department
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3389/fnsyn.2020.00006
