Cell-type Specific Optogenetic Mice for Dissecting Neural Circuitry Function
Name
Feng-Cell-type Specific Optogenetic.pdf
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1.96 MB
Format
Adobe PDF
Checksum (MD5)
36492ecadca55da71602b996aa1da4db
Author(s)
Zhao, Shengli
Ting, Jonathan Thomas
Atallah, Hicham
Qiu, Li
Tan, Jie
Gloss, Bernd
Augustine, George J.
Deisseroth, Karl
Luo, Minmin
Graybiel, Ann M.
Alternative Title
Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function
Date Issued
October 2011
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Zhao, Shengli et al. "Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function." Nature Methods, 2011 ; 8(9): 745–752.
Version
Author's final manuscript
Abstract
Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function, and dysfunction. We used a Bacterial Artificial Chromosome (BAC) transgenic strategy to express Channelrhodopsin2 (ChR2) under the control of cell-type specific promoter elements. We provide a detailed functional characterization of the newly established VGAT-ChR2-EYFP, ChAT-ChR2-EYFP, TPH2-ChR2-EYFP and Pvalb-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action potential firing of GABAergic, cholinergic, serotonergic, and parvalbumin+ neuron subsets using blue light. This resource of cell type-specific ChR2 mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nmeth.1668
