Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing
Name
pDNA Review Final Version - 060911_Accepted_OA.pdf
Description
Accepted version
Size
423.34 KB
Format
Adobe PDF
Checksum (MD5)
9e6e2a3f1e49a14f86008f6136a5ad17
Author(s)
Gonçalves, Geisa A. L.
Bower, Diana Morgan
Prazeres, Duarte M. F.
Monteiro, Gabriel A.
Jones, Kristala L.
Date Issued
September 2011
Journal
Biotechnology Journal
Publisher
Wiley
Citation
Goncalves, Geisa A. L. et al. "Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing." Biotechnology Journal 7, 2 (February 2012): 251-261 © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Version
Author's final manuscript
Abstract
Plasmid DNA (pDNA) has become very attractive as a biopharmaceutical, especially for gene therapy and DNA vaccination. Currently, there are a few products licensed for veterinary applications and numerous plasmids in clinical trials for use in humans. Recent work in both academia and industry demonstrates a need for technological and economical improvement in pDNA manufacturing. Significant progress has been achieved in plasmid design and downstream processing, but there is still a demand for improved production strains. This review focuses on engineering of Escherichia coli strains for plasmid DNA production, understanding the differences between the traditional use of pDNA for recombinant protein production and its role as a biopharmaceutical. We will present recent developments in engineering of E. coli strains, highlight essential genes for improvement of pDNA yield and quality, and analyze the impact of various process strategies on gene expression in pDNA production strains. Keywords: Central metabolism; Escherichia coli; Metabolic engineering; Plasmid biopharmaceuticals; Strain engineering
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/biot.201100062
