High-resolution imaging of cellular dopamine efflux using a fluorescent nanosensor array
Name
Kruss-2017-High-resolution imaging of cellular.pdf
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Author(s)
Kruss, Sebastian
Salem, Daniel Parker
Lima, Barbara M.
Vander Ende, Emma R
Boyden, Edward
Strano, Michael S.
Date Issued
February 2017
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Kruss, Sebastian et al. “High-Resolution Imaging of Cellular Dopamine Efflux Using a Fluorescent Nanosensor Array.” Proceedings of the National Academy of Sciences 114, 8 (February 2017): 1789–1794 © 2017 National Academy of Sciences
Version
Final published version
Abstract
Intercellular communication via chemical signaling proceeds with both spatial and temporal components, but analytical tools, such as microfabricated electrodes, have been limited to just a few probes per cell. In this work, we use a nonphotobleaching fluorescent nanosensor array based on single-walled carbon nanotubes (SWCNTs) rendered selective to dopamine to study its release from PC12 neuroprogenitor cells at a resolution exceeding 20,000 sensors per cell. This allows the spatial and temporal dynamics of dopamine release, following K⁺ stimulation, to be measured at exceedingly high resolution. We observe localized, unlabeled release sites of dopamine spanning 100 ms to seconds that correlate with protrusions but not predominately the positive curvature associated with the tips of cellular protrusions as intuitively expected. The results illustrate how directionality of chemical signaling is shaped by membrane morphology, and highlight the advantages of nanosensor arrays that can provide high spatial and temporal resolution of chemical signaling.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1613541114
