A microneedle vaccine printer for thermostable COVID-19 mRNA vaccines
Name
nihms-1905817.pdf
Description
Accepted version
Size
4.66 MB
Format
Adobe PDF
Checksum (MD5)
c190c73caac10248c68a883fc3e49048
Author(s)
vander Straeten, Aurélien
Sarmadi, Morteza
Daristotle, John L
Kanelli, Maria
Tostanoski, Lisa H
Collins, Joe
Pardeshi, Apurva
Han, Jooli
Varshney, Dhruv
Eshaghi, Behnaz
Date Issued
April 24, 2024
Journal
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Citation
vander Straeten, A., Sarmadi, M., Daristotle, J.L. et al. A microneedle vaccine printer for thermostable COVID-19 mRNA vaccines. Nat Biotechnol 42, 510–517 (2024).
Version
Author's final manuscript
Abstract
Decentralized manufacture of thermostable mRNA vaccines in a microneedle patch (MNP) format could enhance vaccine access in low-resource communities by eliminating the need for a cold chain and trained healthcare personnel. Here we describe an automated process for printing MNP Coronavirus Disease 2019 (COVID-19) mRNA vaccines in a standalone device. The vaccine ink is composed of lipid nanoparticles loaded with mRNA and a dissolvable polymer blend that was optimized for high bioactivity by screening formulations in vitro. We demonstrate that the resulting MNPs are shelf stable for at least 6 months at room temperature when assessed using a model mRNA construct. Vaccine loading efficiency and microneedle dissolution suggest that efficacious, microgram-scale doses of mRNA encapsulated in lipid nanoparticles could be delivered with a single patch. Immunizations in mice using manually produced MNPs with mRNA encoding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein receptor-binding domain stimulate long-term immune responses similar to those of intramuscular administration.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Chemical Engineering
Ragon Institute of MGH, MIT and Harvard
Terms of Use
Creative Commons Attribution-Noncommercial-ShareAlike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41587-023-01774-z
