Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
Name
nihms-1736872.pdf
Description
Accepted version
Size
1.98 MB
Format
Adobe PDF
Checksum (MD5)
01b52613f71022d019c63b3fa6a9e1ec
Author(s)
Segel, Michael
Lash, Blake
Song, Jingwei
Ladha, Alim
Liu, Catherine C
Jin, Xin
Mekhedov, Sergei L
Macrae, Rhiannon K
Koonin, Eugene V
Zhang, Feng
Date Issued
August 20, 2021
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Segel, Michael, Lash, Blake, Song, Jingwei, Ladha, Alim, Liu, Catherine C et al. 2021. "Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery." Science, 373 (6557).
Version
Author's final manuscript
Abstract
Hitching a ride with a retroelement
Retroviruses and retroelements have inserted their genetic code into mammalian genomes throughout evolution. Although many of these integrated virus-like sequences pose a threat to genomic integrity, some have been retooled by mammalian cells to perform essential roles in development. Segel et al . found that one of these retroviral-like proteins, PEG10, directly binds to and secretes its own mRNA in extracellular virus–like capsids. These virus-like particles were then pseudotyped with fusogens to deliver functional mRNA cargos to mammalian cells. This potentially provides an endogenous vector for RNA-based gene therapy. —DJ
Retroviruses and retroelements have inserted their genetic code into mammalian genomes throughout evolution. Although many of these integrated virus-like sequences pose a threat to genomic integrity, some have been retooled by mammalian cells to perform essential roles in development. Segel et al . found that one of these retroviral-like proteins, PEG10, directly binds to and secretes its own mRNA in extracellular virus–like capsids. These virus-like particles were then pseudotyped with fusogens to deliver functional mRNA cargos to mammalian cells. This potentially provides an endogenous vector for RNA-based gene therapy. —DJ
MIT Department
Howard Hughes Medical Institute
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/science.abg6155
