Compact RNA editors with small Cas13 proteins
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nihms-1739709.pdf
Description
Accepted version
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988.9 KB
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Author(s)
Kannan, Soumya
Altae-Tran, Han
Jin, Xin
Madigan, Victoria J
Oshiro, Rachel
Makarova, Kira S
Koonin, Eugene V
Zhang, Feng
Date Issued
February 2022
Journal
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Citation
Kannan, Soumya, Altae-Tran, Han, Jin, Xin, Madigan, Victoria J, Oshiro, Rachel et al. 2022. "Compact RNA editors with small Cas13 proteins." Nature Biotechnology, 40 (2).
Version
Author's final manuscript
Abstract
CRISPR-Cas13 systems have been developed for precise RNA editing, and can potentially be used therapeutically when temporary changes are desirable or when DNA editing is challenging. We have identified and characterized an ultrasmall family of Cas13b proteins-Cas13bt-that can mediate mammalian transcript knockdown. We have engineered compact variants of REPAIR and RESCUE RNA editors by functionalizing Cas13bt with adenosine and cytosine deaminase domains, and demonstrated packaging of the editors within a single adeno-associated virus.
MIT Department
Howard Hughes Medical Institute
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/s41587-021-01030-2
