Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region
Name
nihms-1024025.pdf
Description
Accepted version
Size
2 MB
Format
Adobe PDF
Checksum (MD5)
b6442d826f9a4a9314a6a0c3efa88a66
Author(s)
Regev, Aviv
Date Issued
November 2018
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Moffitt, Jeffrey R. et al. “Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region.” Science 362 (2018): eaau5324 © 2018 The Author(s)
Version
Author's final manuscript
Abstract
The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei—including the molecular identity, spatial organization, and function of distinct cell types—is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. We profiled ~1 million cells, identified ~70 neuronal populations characterized by distinct neuromodulatory signatures and spatial organizations, and defined specific neuronal populations activated during social behaviors in male and female mice, providing a high-resolution framework for mechanistic investigation of behavior circuits. The approach described opens a new avenue for the construction of cell atlases in diverse tissues and organisms.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/SCIENCE.AAU5324
