Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute-phase Toxoplasma gondii burden
Name
Sanecka_et_al-2016-Immunology.pdf
Description
Published version
Size
1.04 MB
Format
Adobe PDF
Checksum (MD5)
280f353636649b58c8b4fc45265069cc
Author(s)
Sanecka, Anna
Yoshida, Nagisa
Dougan, Stephanie K.
Jackson, John
Shastri, Nilabh
Ploegh, Hidde
Blanchard, Nicolas
Frickel, Eva-Maria
Date Issued
July 2016
Journal
Immunology
Publisher
Wiley
Citation
Sanecka, Anna et al. "Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute-phase Toxoplasma gondii burden." Immunology 149 (July 2016): 270-279 © 2016 The Authors
Version
Final published version
Abstract
We generated a CD8 T-cell receptor (TCR) transnuclear (TN) mouse specific to the Ld-restricted immunodominant epitope of GRA6 from Toxoplasma gondii as a source of cells to facilitate further investigation into the CD8 T-cell-mediated response against this pathogen. The TN T cells bound Ld-Gra6 tetramer and proliferated upon unspecific and peptide-specific stimulation. The TCR beta sequence of the Gra6-specific TN CD8 T cells is identical in its V- and J-region to the TCR-β harboured by a hybridoma line generated in response to Gra6 peptide. Adoptively transferred Gra6 TN CD8 T cells proliferated upon Toxoplasma infection in vivo and exhibited an activated phenotype similar to host CD8 T cells specific to Gra6. The brain of Toxoplasma-infected mice carried Gra6 TN cells already at day 8 post-infection. Both Gra6 TN mice as well as adoptively transferred Gra6 TN cells were able to significantly reduce the parasite burden in the acute phase of Toxoplasma infection. Overall, the Gra6 TN mouse represents a functional tool to study the protective and immunodominant specific CD8 T-cell response to Toxoplasma in both the acute and the chronic phases of infection. Keywords: antigens/peptides/epitopes; parasitic protozoan; T cells
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1111/IMM.12643
