Genetic Circuits in Salmonella typhimurium
Name
Bhatia_Genetic circuits.pdf
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1.21 MB
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Author(s)
Prindle, Arthur
Selimkhanov, Jangir
Danino, Tal
Samayoa, Phillip
Goldber, Anna
Hasty, Jeff
Bhatia, Sangeeta N
Date Issued
August 2012
Journal
ACS Synthetic Biology
Publisher
American Chemical Society (ACS)
Citation
Prindle, Arthur et al. “Genetic Circuits in Salmonella Typhimurium.” ACS Synthetic Biology 1.10 (2012): 458–464. Copyright © 2012 American Chemical Society
Version
Final published version
Abstract
Synthetic biology has rapidly progressed over the
past decade and is now positioned to impact important problems
in health and energy. In the clinical arena, the field has thus far
focused primarily on the use of bacteria and bacteriophages to
overexpress therapeutic gene products. The next generation of
multigene circuits will control the triggering, amplitude, and
duration of therapeutic activity in vivo. This will require a host
organism that is easy to genetically modify, leverages existing
successful circuit designs, and has the potential for use in humans.
Here, we show that gene circuits that were originally constructed
and tested in Escherichia coli translate to Salmonella typhimurium, a
therapeutically relevant microbe with attenuated strains that have
exhibited safety in several human clinical trials. These strains are essentially nonvirulent, easy to genetically program, and
specifically grow in tumor environments. Developing gene circuits on this platform could enhance our ability to bring
sophisticated genetic programming to cancer therapy, setting the stage for a new generation of synthetic biology in clinically
relevant microbes.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/sb300060e
