Janus Emulsions for the Detection of Bacteria
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acscentsci.7b00021.pdf
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3.38 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Kaplonek, Paulina
Seeberger, Peter H.
Zhang, Qifan
Savagatrup, Suchol
Swager, Timothy M
Date Issued
March 2017
Journal
ACS Central Science
Publisher
American Chemical Society (ACS)
Citation
Zhang, Qifan et al. “Janus Emulsions for the Detection of Bacteria.” ACS Central Science 3, 4 (March 2017): 309–313 © 2017 American Chemical Society
Version
Final published version
Abstract
Janus emulsion assays that rely on carbohydrate-lectin binding for the detection of Escherichia coli bacteria are described. Surfactants containing mannose are self-assembled at the surface of Janus droplets to produce particles with lectin binding sites. Janus droplets orient in a vertical direction as a result of the difference in densities between the hydrocarbon and fluorocarbon solvents. Binding of lectin to mannose(s) causes agglutination and a tilted geometry. The distinct optical difference between naturally aligned and agglutinated Janus droplets produces signals that can be detected quantitatively. The Janus emulsion assay sensitively and selectively binds to E. coli at 10 4 cfu/mL and can be easily prepared with long-time stability. It provides the basis for the development of inexpensive portable devices for fast, on-site pathogen detection.
MIT Department
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Massachusetts Institute of Technology. Department of Chemistry
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ACSCENTSCI.7B00021
