Efficient retroelement-mediated DNA writing in bacteria
Name
2020.02.21.958983v1.full.pdf
Description
Submitted version
Size
2.59 MB
Format
Unknown
Checksum (MD5)
187d26f0ebf5d193d5555d5061de3643
Author(s)
Farzadfard, Fahim
Gharaei, Nava
Citorik, Robert J.
Lu, Timothy K.
Date Issued
September 2021
Journal
Cell Systems
Publisher
Elsevier BV
Citation
Farzadfard, Fahim, Gharaei, Nava, Citorik, Robert J and Lu, Timothy K. 2021. "Efficient retroelement-mediated DNA writing in bacteria." Cell Systems, 12 (9).
Version
Original manuscript
Abstract
The ability to efficiently and dynamically change information stored in genomes would enable powerful strategies for studying cell biology and controlling cellular phenotypes. Current recombineering-mediated DNA writing platforms in bacteria are limited to specific laboratory conditions, often suffer from suboptimal editing efficiencies, and are not suitable for in situ applications. To overcome these limitations, we engineered a retroelement-mediated DNA writing system that enables efficient and precise editing of bacterial genomes without the requirement for target-specific elements or selection. We demonstrate that this DNA writing platform enables a broad range of applications, including efficient, scarless, and cis-element-independent editing of targeted microbial genomes within complex communities, the high-throughput mapping of spatial information and cellular interactions into DNA memory, and the continuous evolution of cellular traits.
MIT Department
Massachusetts Institute of Technology. Synthetic Biology Center
Massachusetts Institute of Technology. Research Laboratory of Electronics
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Microbiology Graduate Program
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.cels.2021.07.001
