Extensive changes in DNA methylation are associated with expression of mutant huntingtin
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Ng-2013-Extensive changes in.pdf
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Author(s)
Ng, Christopher W.
Yildirim, Ferah
Yap, Soon Ming Micha
Matthews, Bryan
Velez, Patricio J.
Labadorf, Adam
Fraenkel, Ernest
Dalin, Simona
Housman, David E
Date Issued
January 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Ng, C. W., F. Yildirim, Y. S. Yap, S. Dalin, B. J. Matthews, P. J. Velez, A. Labadorf, D. E. Housman, and E. Fraenkel. “Extensive changes in DNA methylation are associated with expression of mutant huntingtin.” Proceedings of the National Academy of Sciences 110, no. 6 (February 5, 2013): 2354-2359.
Version
Final published version
Abstract
The earliest stages of Huntington disease are marked by changes in gene expression that are caused in an indirect and poorly understood manner by polyglutamine expansions in the huntingtin (HTT) protein. To explore the hypothesis that DNA methylation may be altered in cells expressing mutated HTT, we use reduced representation bisulfite sequencing (RRBS) to map sites of DNA methylation in cells carrying either wild-type or mutant HTT. We find that a large fraction of the genes that change in expression in the presence of mutant huntingtin demonstrate significant changes in DNA methylation. Regions with low CpG content, which have previously been shown to undergo methylation changes in response to neuronal activity, are disproportionately affected. On the basis of the sequence of regions that change in methylation, we identify AP-1 and SOX2 as transcriptional regulators associated with DNA methylation changes, and we confirm these hypotheses using genome-wide chromatin immunoprecipitation sequencing (ChIP-Seq). Our findings suggest new mechanisms for the effects of polyglutamine-expanded HTT. These results also raise important questions about the potential effects of changes in DNA methylation on neurogenesis and cognitive decline in patients with Huntington disease.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1073/pnas.1221292110
