Fluorescent Janus emulsions for biosensing ofListeria monocytogenes
Name
11923.full.pdf
Description
Published version
Size
2.17 MB
Format
Adobe PDF
Checksum (MD5)
e1a545772da4abd3a5805597fd8e64f9
Author(s)
Li, Jie
Savagatrup, Suchol
Nelson, Zachary
Yoshinaga, Kosuke
Swager, Timothy M
Date Issued
May 2020
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
Proceedings of the National Academy of Sciences
Citation
Li, Jie et al. "Fluorescent Janus emulsions for biosensing of Listeria monocytogenes." Proceedings of the National Academy of Sciences 117, 22 (June 2020): 11923-11930
Version
Final published version
Abstract
Here we report a sensing method for Listeria monocytogenes based on the agglutination of all-liquid Janus emulsions. This two-dye assay enables the rapid detection of trace Listeria in less than 2 h via an emissive signal produced in response to Listeria binding. The biorecognition interface between the Janus emulsions is assembled by attaching antibodies to a functional surfactant polymer with a tetrazine/transcyclooctene click reaction. The strong binding between Listeria and the Listeria antibody located at the hydrocarbon surface of the emulsions results in the tilting of the Janus structure from its equilibrium position to produce emission that would ordinarily be obscured by a blocking dye. This method provides rapid and inexpensive Listeria detection with high sensitivity (<100 CFU/mL in 2 h) that can be paired with many antibody or related recognition elements to create a new class of biosensors.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.2002623117
