DNA phosphorothioation is widespread and quantized in bacterial genomes
Name
Dedon-2011-DNA phosphorothioation.pdf
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504.97 KB
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Checksum (MD5)
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Author(s)
Wang, Lianrong
Chen, Shi
Vergin, Kevin L.
Giovannoni, Stephen J.
Chan, Simon W.
DeMott, Michael S.
Taghizadeh, Koli
Cordero Sanchez, Otto Xavier
Cutler, Michael Barry
Timberlake, Sonia
Date Issued
February 2011
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences
Citation
Wang, L. et al. “DNA Phosphorothioation Is Widespread and Quantized in Bacterial Genomes.” Proceedings of the National Academy of Sciences 108.7 (2011) : 2963-2968. Copyright ©2011 by the National Academy of Sciences
Version
Final published version
Abstract
Phosphorothioate (PT) modification of DNA, with sulfur replacing a nonbridging phosphate oxygen, was recently discovered as a product of the dnd genes found in bacteria and archaea. Given our limited understanding of the biological function of PT modifications, including sequence context, genomic frequencies, and relationships to the diversity of dnd gene clusters, we undertook a quantitative study of PT modifications in prokaryotic genomes using a liquid chromatography-coupled tandem quadrupole mass spectrometry approach. The results revealed a diversity of unique PT sequence contexts and three discrete genomic frequencies in a wide range of bacteria. Metagenomic analyses of PT modifications revealed unique ecological distributions, and a phylogenetic comparison of dnd genes and PT sequence contexts strongly supports the horizontal transfer of dnd genes. These results are consistent with the involvement of PT modifications in a type of restriction-modification system with wide distribution in prokaryotes.
MIT Department
Massachusetts Institute of Technology. Center for Environmental Health Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1017261108
