Automated fast-flow synthesis of the immune checkpoint receptors PD-1 and PD-L1
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Author(s)
Fittolani, Giulio
Callahan, Alex J.
Loas, Andrei
Pentelute, Bradley L.
Date Issued
March 17, 2025
Journal
Chemical Communications
Publisher
Royal Society of Chemistry
Citation
Fittolani, Giulio, Callahan, Alex J., Loas, Andrei and Pentelute, Bradley L. 2025. "Automated fast-flow synthesis of the immune checkpoint receptors PD-1 and PD-L1." Chemical Communications, (29).
Version
Final published version
Abstract
Programmed cell death protein 1 (PD-1) and programmed cell death ligand 1 (PD-L1) are key targets for cancer therapy. Here, we use automated fast-flow peptide synthesis (AFPS) to rapidly produce these challenging β-sheet-rich proteins in their active forms following oxidative refolding protocols. The methods presented here provide rapid access to synthetic, air-stable mutants of PD-1 and PD-L1 in which L-methionine residues are substituted with L-norleucine, potentially enabling investigation of post-translational modifications and mirror-image analogs for drug discovery.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Center for Environmental Health Sciences
Broad Institute of MIT and Harvard
Terms of Use
Creative Commons Attribution-Noncommercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/D4CC05982D
