Imaging stress
Name
12192_2015_Article_615.pdf
Size
637.55 KB
Format
Adobe PDF
Checksum (MD5)
8f788daf49d85cbb802e5fc317bd5d84
Author(s)
Brielle, Shlomi
Kaganovich, Daniel
Gura Sadovsky, Rotem
Date Issued
July 2015
Journal
Cell Stress and Chaperones
Publisher
Springer Netherlands
Citation
Brielle, Shlomi, Rotem Gura, and Daniel Kaganovich. “Imaging Stress.” Cell Stress and Chaperones 20, no. 6 (July 4, 2015): 867–874.
Version
Author's final manuscript
Abstract
Recent innovations in cell biology and imaging approaches are changing the way we study cellular stress, protein misfolding, and aggregation. Studies have begun to show that stress responses are even more variegated and dynamic than previously thought, encompassing nano-scale reorganization of cytosolic machinery that occurs almost instantaneously, much faster than transcriptional responses. Moreover, protein and mRNA quality control is often organized into highly dynamic macromolecular assemblies, or dynamic droplets, which could easily be mistaken for dysfunctional “aggregates,” but which are, in fact, regulated functional compartments. The nano-scale architecture of stress-response ranges from diffraction-limited structures like stress granules, P-bodies, and stress foci to slightly larger quality control inclusions like juxta nuclear quality control compartment (JUNQ) and insoluble protein deposit compartment (IPOD), as well as others. Examining the biochemical and physical properties of these dynamic structures necessitates live cell imaging at high spatial and temporal resolution, and techniques to make quantitative measurements with respect to movement, localization, and mobility. Hence, it is important to note some of the most recent observations, while casting an eye towards new imaging approaches that offer the possibility of collecting entirely new kinds of data from living cells.
MIT Department
Massachusetts Institute of Technology. Computational and Systems Biology Program
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1007/s12192-015-0615-y
