Neuronal activity drives pathway-specific depolarization of peripheral astrocyte processes
Name
nihms-1788777.pdf
Description
Accepted version
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5.26 MB
Format
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Checksum (MD5)
470b63aaaace649fc0e1cc3aa4a9c20f
Author(s)
Armbruster, Moritz
Naskar, Saptarnab
Garcia, Jacqueline P
Sommer, Mary
Kim, Elliot
Adam, Yoav
Haydon, Philip G
Boyden, Edward S
Cohen, Adam E
Dulla, Chris G
Date Issued
2022
Journal
Nature Neuroscience
Publisher
Springer Science and Business Media LLC
Citation
Armbruster, Moritz, Naskar, Saptarnab, Garcia, Jacqueline P, Sommer, Mary, Kim, Elliot et al. 2022. "Neuronal activity drives pathway-specific depolarization of peripheral astrocyte processes." Nature Neuroscience, 25 (5).
Version
Author's final manuscript
Abstract
Astrocytes are glial cells that interact with neuronal synapses via their distal processes, where they remove glutamate and potassium (K+) from the extracellular space following neuronal activity. Astrocyte clearance of both glutamate and K+ is voltage dependent, but astrocyte membrane potential (Vm) is thought to be largely invariant. As a result, these voltage dependencies have not been considered relevant to astrocyte function. Using genetically encoded voltage indicators to enable the measurement of Vm at peripheral astrocyte processes (PAPs) in mice, we report large, rapid, focal and pathway-specific depolarizations in PAPs during neuronal activity. These activity-dependent astrocyte depolarizations are driven by action potential-mediated presynaptic K+ efflux and electrogenic glutamate transporters. We find that PAP depolarization inhibits astrocyte glutamate clearance during neuronal activity, enhancing neuronal activation by glutamate. This represents a novel class of subcellular astrocyte membrane dynamics and a new form of astrocyte-neuron interaction.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/S41593-022-01049-X
