Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities
Name
nihms542918.pdf
Size
1.55 MB
Format
Adobe PDF
Checksum (MD5)
a082e21804e8de31fda3e748d3fcd252
Author(s)
Leung, Anthony K. L.
Sharp, Phillip A.
Date Issued
2013
Journal
Advances in Experimental Medicine and Biology: Ten Years of Progress in GW/P Body Research
Publisher
Springer
Citation
Leung, Anthony K. L. and Phillip A. Sharp. "Quantifying argonaute proteins in and out of GW/P-bodies: Implications in microRNA activities." Advances in Experimental Medicine and Biology: Ten Years of Progress in GW/P Body Research, edited by E. Chan and M. Fritzler, Springer, 2013: 165-182 © Springer Science+Business Media New York 2013.
Version
Author's final manuscript
Abstract
MicroRNAs (miRNAs) are a class of ∼22nt non-coding RNAs that regulate the translational potential and stability of mRNAs. Though constituting only 1-4% of human genes, miRNAs are predicted to regulate more than 60% of all mRNAs. The action of miRNAs is mediated through their associations with Argonaute proteins and mRNA targets. Previous studies indicated that though the majority of Argonaute proteins is diffusely distributed in the cytoplasm, a small fraction is consistently observed to be concentrated in a cytoplasmic compartment called GW/P-bodies. In this chapter, we will provide a quantitative and dynamic view of the subcellular localization of miRNA function, followed by a discussion on the possible roles of PBs in miRNA silencing.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1007/978-1-4614-5107-5_10
