Formation of a “Pre-mouth Array” from the Extreme Anterior Domain Is Directed by Neural Crest and Wnt/PCP Signaling
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Jacox-2016-Formation of a _Pre-.pdf
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Author(s)
Lathrop-Marshall, Hillary
Chen, Justin
Rothman, Alyssa L.
Jacox, Laura A.
Sive, Hazel L.
Date Issued
July 2016
Journal
Cell Reports
Publisher
Elsevier
Citation
Jacox, Laura et al. “Formation of a ‘Pre-Mouth Array’ from the Extreme Anterior Domain Is Directed by Neural Crest and Wnt/PCP Signaling.” Cell Reports 16.5 (2016): 1445–1455.
Version
Final published version
Abstract
The mouth arises from the extreme anterior domain (EAD), a region where the ectoderm and endoderm are directly juxtaposed. Here, we identify a ‘‘premouth array’’ in Xenopus that forms soon after the cranial neural crest has migrated to lie on either side of the EAD. Initially, EAD ectoderm comprises a wide and short epithelial mass that becomes narrow and tall with cells and nuclei changing shape, a characteristic of convergent extension. The resulting two rows of cells—the pre-mouth array—later split
down the midline to surround the mouth opening. Neural crest is essential for convergent extension and likely signals to the EAD through the Wnt/planar
cell polarity (PCP) pathway. Fzl7 receptor is locally required in EAD ectoderm, while Wnt11 ligand is required more globally. Indeed, heterologous cells expressing Wnt11 can elicit EAD convergent extension. The study reveals a precise cellular mechanism that positions and contributes to the future mouth.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2016.06.073
