Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions
Name
Miller-2012-Meiosis I chromosome.pdf
Size
3.97 MB
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Adobe PDF
Checksum (MD5)
ae962c346ec6d8c981ad710bef0f99d2
Author(s)
Miller, Matthew P.
Unal, Elcin
Brar, Gloria A.
Amon, Angelika B
Date Issued
December 2012
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Miller, Matthew P, Elçin Ünal, Gloria A Brar, and Angelika Amon. “Meiosis I Chromosome Segregation Is Established through Regulation of Microtubule–kinetochore Interactions.” eLife;1:e00117 (December 18, 2012).
Version
Final published version
Abstract
During meiosis, a single round of DNA replication is followed by two consecutive rounds of nuclear divisions called meiosis I and meiosis II. In meiosis I, homologous chromosomes segregate, while sister chromatids remain together. Determining how this unusual chromosome segregation behavior is established is central to understanding germ cell development. Here we show that preventing microtubule–kinetochore interactions during premeiotic S phase and prophase I is essential for establishing the meiosis I chromosome segregation pattern. Premature interactions of kinetochores with microtubules transform meiosis I into a mitosis-like division by disrupting two key meiosis I events: coorientation of sister kinetochores and protection of centromeric cohesin removal from chromosomes. Furthermore we find that restricting outer kinetochore assembly contributes to preventing premature engagement of microtubules with kinetochores. We propose that inhibition of microtubule–kinetochore interactions during premeiotic S phase and prophase I is central to establishing the unique meiosis I chromosome segregation pattern.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.7554/eLife.00117
