Mitigation of resource competition in synthetic genetic circuits through feedback regulation
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Amadeh_cdc_final.pdf
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178.87 KB
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Author(s)
Hamadeh, Abdullah
Del Vecchio, Domitilla
Date Issued
December 2014
Journal
2014 IEEE 53rd Conference on Decision and Control (CDC)
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Citation
Hamadeh, Abdullah, and Domitilla Del Vecchio. “Mitigation of Resource Competition in Synthetic Genetic Circuits through Feedback Regulation.” 53rd IEEE Conference on Decision and Control (December 2014), Los Angeles, CA, USA, Institute of Electrical and Electronics Engineers (IEEE), 2014.
Version
Author's final manuscript
Abstract
A recurring challenge in the design of genetic circuits is context dependence, the fact that the behavior of a functional module is influenced by the state of the wider cellular milieu with which it interacts. One key player in context dependence is the scarcity of shared cellular resources, especially those required for transcription and translation during gene expression. Because of competition for these limited resources, the behavior of modules becomes coupled in subtle ways, preventing circuits from working as expected. This paper proposes a classical feedback control approach to mitigate the steady state effects of the competition for resources necessary for gene expression. In particular, we analyze and compare the ability of several inhibitory feedback regulation architectures to reduce the interdependence between different gene expression processes due to resource limitations.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1109/CDC.2014.7039985
