Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice
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1471-213X-4-2.pdf
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Author(s)
Bortvin, Alex
Goodheart, Mary L.
Liao, Michelle
Page, David C.
Date Issued
February 2004
Journal
BMC Developmental Biology
Publisher
BioMed Central Ltd
Citation
Bortvin, Alex, Mary Goodheart, Michelle Liao, and David C. Page (2004). Dppa3 / Pgc7 / stellais a maternal factor and is not required for germ cell specification in mice. BMC developmental biology 4:2/1-15.
Version
Final published version
Abstract
Background: In mice, germ cells are specified through signalling between layers of cells comprising the primitive embryo. The function of Dppa3 (also known as Pgc7 or stella), a gene expressed in primordial germ cells at the time of their emergence in gastrulating embryos, is unknown, but a recent study has claimed that it plays a central role in germ cell specification. Results: To test Dppa3's role in germ cell development, we disrupted the gene in mouse embryonic stem cells and generated mutant animals. We were able to obtain viable and fertile Dppa3-deficient animals of both sexes. Examination of embryonic and adult germ cells and gonads in Dppa3-deficient animals did not reveal any defects. However, most embryos derived from Dppa3-deficient oocytes failed to develop normally beyond the four-cell stage. Conclusion: We found that Dppa3 is an important maternal factor in the cleavage stages of mouse embryogenesis. However, it is not required for germ cell specification.
Subjects
Animals
Biological factors, physiology
Cell differentiation, physiology
Embryo, mammalian, cytology
Embryo, mammalian, physiology
Female
Gene expression regulation, developmental, physiology
Germ cells, physiology
Gonads, cytology
Gonads, physiology
Male
Mice
Ovary, cytology
Ovary, physiology
Proteins, physiology
Repressor proteins, physiology
Biological factors
Dppa3 protein, mouse
Proteins
Repressor proteins
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1186/1471-213X-4-2
