Helminth co-infection in Helicobacter pylori infected INS-GAS mice attenuates gastric premalignant lesions of epithelial dysplasia and glandular atrophy and preserves colonization resistance of the stomach to lower bowel microbiota
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Author(s)
Whary, Mark T.
Muthupalani, Sureshkumar
Ge, Zhongming
Feng, Yan
Lofgren, Jennifer L.
Shi, Hai Ning
Taylor, Nancy S.
Correa, Pelayo
Versalovic, James
Wang, Timothy C.
Date Issued
February 2014
Journal
Microbes and Infection
Publisher
Elsevier
Citation
Whary, Mark T., Sureshkumar Muthupalani, Zhongming Ge, Yan Feng, Jennifer Lofgren, Hai Ning Shi, Nancy S. Taylor, et al. “Helminth Co-Infection in Helicobacter Pylori Infected INS-GAS Mice Attenuates Gastric Premalignant Lesions of Epithelial Dysplasia and Glandular Atrophy and Preserves Colonization Resistance of the Stomach to Lower Bowel Microbiota.” Microbes and Infection 16, no. 4 (April 2014): 345–55.
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Author's final manuscript
Abstract
Higher prevalence of helminth infections in Helicobacter pylori infected children was suggested to potentially lower the life-time risk for gastric adenocarcinoma. In rodent models, helminth co-infection does not reduce Helicobacter-induced inflammation but delays progression of pre-malignant gastric lesions. Because gastric cancer in INS-GAS mice is promoted by intestinal microflora, the impact of Heligmosomoides polygyrus co-infection on H. pylori-associated gastric lesions and microflora were evaluated. Male INS-GAS mice co-infected with H. pylori and H. polygyrus for 5 months were assessed for gastrointestinal lesions, inflammation-related mRNA expression, FoxP3[superscript +] cells, epithelial proliferation, and gastric colonization with H. pylori and Altered Schaedler Flora. Despite similar gastric inflammation and high levels of proinflammatory mRNA, helminth co-infection increased FoxP3[superscript +] cells in the corpus and reduced H. pylori-associated gastric atrophy (p < 0.04), dysplasia (p < 0.02) and prevented H. pylori-induced changes in the gastric flora (p < 0.05). This is the first evidence of helminth infection reducing H. pylori-induced gastric lesions while inhibiting changes in gastric flora, consistent with prior observations that gastric colonization with enteric microbiota accelerated gastric lesions in INS-GAS mice. Identifying how helminths reduce gastric premalignant lesions and impact bacterial colonization of the H. pylori infected stomach could lead to new treatment strategies to inhibit progression from chronic gastritis to cancer in humans.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Division of Comparative Medicine
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DOI of Published Version
https://doi.org/10.1016/j.micinf.2014.01.005
