Visualization of Mismatch Repair in Bacterial Cells
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Grossman_Visualization of mismatch.pdf
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332.32 KB
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Author(s)
Smith, Bradley T.
Walker, Graham C.
Grossman, Alan Davis
Date Issued
December 2001
Journal
Molecular Cell
Publisher
Elsevier
Citation
Smith, Bradley T, Alan D Grossman, and Graham C Walker. “Visualization of Mismatch Repair in Bacterial Cells.” Molecular Cell 8.6 (2001): 1197–1206. Copyright © 2001 Cell Press
Version
Final published version
Abstract
We determined the localizations of mismatch repair proteins in living Bacillus subtilis cells. MutS-GFP colocalized with the chromosome in all cells and formed foci in a subset of cells. MutL-GFP formed foci in a subset of cells, and its localization was MutS dependent. The introduction of mismatches by growth in 2-aminopurine caused a replication-dependent increase in the number of cells with MutS and MutL foci. Approximately half of the MutS foci colocalized with DNA polymerase foci. We conclude that MutS is associated with the entire chromosome, poised to detect mismatches. After detection, it appears that mismatch repair foci assemble at mismatches as they emerge from the DNA polymerase and are then carried away from the replisome by continuing replication.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/S1097-2765(01)00402-6
