Large-Scale Analysis of CRISPR/Cas9 Cell-Cycle Knockouts Reveals the Diversity of p53-Dependent Responses to Cell-Cycle Defects
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nihms847196.pdf
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2.98 MB
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Author(s)
McKinley, Kara Lavidge
Cheeseman, Iain M
Date Issued
February 2017
Journal
Developmental Cell
Publisher
Elsevier BV
Citation
McKinley, Kara L. and Iain M. Cheeseman. “Large-Scale Analysis of CRISPR/Cas9 Cell-Cycle Knockouts Reveals the Diversity of P53-Dependent Responses to Cell-Cycle Defects.” Developmental Cell 40, 4 (February 2017): 405–420 © 2017 Elsevier Inc
Version
Author's final manuscript
Abstract
Defining the genes that are essential for cellular proliferation is critical for understanding organismal development and identifying high-value targets for disease therapies. However, the requirements for cell-cycle progression in human cells remain incompletely understood. To elucidate the consequences of acute and chronic elimination of cell-cycle proteins, we generated and characterized inducible CRISPR/Cas9 knockout human cell lines targeting 209 genes involved in diverse cell-cycle processes. We performed single-cell microscopic analyses to systematically establish the effects of the knockouts on subcellular architecture. To define variations in cell-cycle requirements between cultured cell lines, we generated knockouts across cell lines of diverse origins. We demonstrate that p53 modulates the phenotype of specific cell-cycle defects through distinct mechanisms, depending on the defect. This work provides a resource to broadly facilitate robust and long-term depletion of cell-cycle proteins and reveals insights into the requirements for cell-cycle progression. Keywords:
kinetochore; centromere; mitosis; DNA replication; spindle; microtubule; multipolarity; p53; CRISPR/Cas9
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.DEVCEL.2017.01.012
