Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient
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Suresh_Optical measurement.pdf
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Author(s)
Byun, HeeSu
Hillman, Timothy R.
Higgins, John M.
Diez-Silva, Monica
Peng, Zhangli
Dao, Ming
Suresh, Subra
Park, YongKeun
Dasari, Ramachandra Rao
Date Issued
July 2012
Journal
Acta Biomaterialia
Publisher
Elsevier
Citation
Byun, HeeSu, Timothy R. Hillman, John M. Higgins, Monica Diez-Silva, Zhangli Peng, Ming Dao, Ramachandra R. Dasari, Subra Suresh, and YongKeun Park. “Optical Measurement of Biomechanical Properties of Individual Erythrocytes from a Sickle Cell Patient.” Acta Biomaterialia 8, no. 11 (November 2012): 4130–4138.
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Author's final manuscript
Abstract
Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microvasculature. A major challenge in the study of SCD involves accurate characterization of the biomechanical properties of individual RBCs with minimum sample perturbation. Here we report the biomechanical properties of individual RBCs from a SCD patient using a non-invasive laser interferometric technique. We optically measure the dynamic membrane fluctuations of RBCs. The measurements are analyzed with a previously validated membrane model to retrieve key mechanical properties of the cells: bending modulus; shear modulus; area expansion modulus; and cytoplasmic viscosity. We find that high cytoplasmic viscosity at ambient oxygen concentration is principally responsible for the significantly decreased dynamic membrane fluctuations in RBCs with SCD, and that the mechanical properties of the membrane cortex of irreversibly sickled cells (ISCs) are different from those of the other types of RBCs in SCD.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
https://doi.org/10.1016/j.actbio.2012.07.011
