Mammalian cell growth dynamics in mitosis
Name
elife-44700-v2.pdf
Description
Published version
Size
17.77 MB
Format
Adobe PDF
Checksum (MD5)
2f720a19614047e2e9114faae7b41207
Author(s)
Miettinen, Teemu P
Kang, Joon Ho
Yang, Lucy F.
Manalis, Scott R
Date Issued
May 2019
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Miettinen et al. "Mammalian cell growth dynamics in mitosis." eLife 8: e44700 © 2019 The Author(s)
Version
Final published version
Abstract
The extent and dynamics of animal cell biomass accumulation during mitosis are unknown, primarily because growth has not been quantified with sufficient precision and temporal resolution. Using the suspended microchannel resonator and protein synthesis assays, we quantify mass accumulation and translation rates between mitotic stages on a single-cell level. For various animal cell types, growth rates in prophase are commensurate with or higher than interphase growth rates. Growth is only stopped as cells approach metaphase-to-anaphase transition and growth resumes in late cytokinesis. Mitotic arrests stop growth independently of arresting mechanism. For mouse lymphoblast cells, growth in prophase is promoted by CDK1 through increased phosphorylation of 4E-BP1 and cap-dependent protein synthesis. Inhibition of CDK1- driven mitotic translation reduces daughter cell growth. Overall, our measurements counter the traditional dogma that growth during mitosis is negligible and provide insight into antimitotic cancer chemotherapies.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.7554/elife.44700
