Simultaneous whole‐animal 3D imaging of neuronal activity using light‐field microscopy
Name
CBMM-Memo-016.pdf
Size
1.24 MB
Format
Adobe PDF
Checksum (MD5)
db0e40e0a9a9bc9b9151a71c5fdeb198
Author(s)
Prevedel, Robert
Yoon, Young-Gyu
Hoffman, Maximilian
Pak, Nikita
Wetzstein, Gordon
Kato, Saul
Schrödel, Tina
Raskar, Ramesh
Zimmer, Manuel
Boyden, Edward S.
Date Issued
May 18, 2014
Publisher
Center for Brains, Minds and Machines (CBMM)
Series/Report no.
CBMM Memo Series;016
Abstract
High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Description
Notes: Robert Prevedel*, Young‐Gyu Yoon*, Maximilian Hoffmann, Nikita Pak, Gordon Wetzstein, Saul Kato, Tina Schrödel, Ramesh Raskar, Manuel Zimmer, Edward S Boyden** & Alipasha Vaziri** (* equal contributions, ** co-corresponding authors)
Subjects
Microscopy
Neuroscience
Terms of Use
Attribution-NonCommercial 3.0 United States
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