Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors
Name
Cao-2011-Rapid Estimation of.pdf
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380.86 KB
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Author(s)
Cao, Yang
Koh, Xiaoying
Dong, Libo
Du, Xiangjun
Wu, Aiping
Ding, Xilai
Deng, Hongyu
Shu, Yuelong
Chen, Jianzhu
Jiang, Taijiao
Date Issued
April 2011
Journal
PLoS One
Publisher
Public Library of Science
Citation
Cao, Yang et al. “Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors.” Ed. Christian Schönbach. PLoS ONE 6.4 (2011) : e18664.
Version
Final published version
Abstract
A critical step for avian influenza viruses to infect human hosts and cause epidemics or pandemics is acquisition of the ability of the viral hemagglutinin (HA) to bind to human receptors. However, current global influenza surveillance does not monitor HA binding specificity due to a lack of rapid and reliable assays. Here we report a computational method that uses an effective scoring function to quantify HA-receptor binding activities with high accuracy and speed. Application of this method reveals receptor specificity changes and its temporal relationship with antigenicity changes during the evolution of human H3N2 viruses. The method predicts that two amino acid differences at 222 and 225 between HAs of A/Fujian/411/02 and A/Panama/2007/99 viruses account for their differences in binding to both avian and human receptors; this prediction was verified experimentally. The new computational method could provide an urgently needed tool for rapid and large-scale analysis of HA receptor specificities for global influenza surveillance.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0018664
