Cellular resolution ex vivo imaging of gastrointestinal tissues with coherence microscopy
Name
Aguirre-2010-Cellular resolution.pdf
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1.57 MB
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Checksum (MD5)
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Author(s)
Fujimoto, James G.
Aguirre, Aaron Dominic
Chen, Yu
Bryan, Bradley
Mashimo, Hiroshi
Huang, Qin
Connolly, James L.
Date Issued
March 2010
Journal
Journal of Biomedical Optics
Publisher
Society of Photo-optical Instrumentation Engineers
Citation
Aguirre, Aaron D. et al. “Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy.” Journal of Biomedical Optics 15.1 (2010): 016025-9. ©2010 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
Optical coherence microscopy (OCM) combines confocal microscopy and optical coherence tomography (OCT) to improve imaging depth and contrast, enabling cellular imaging in human tissues. We aim to investigate OCM for ex vivo imaging of upper and lower gastrointestinal tract tissues, to establish correlations between OCM imaging and histology, and to provide a baseline for future endoscopic studies. Co-registered OCM and OCT imaging were performed on fresh surgical specimens and endoscopic biopsy specimens, and images were correlated with histology. Imaging was performed at 1.06-µm wavelength with <2-µm transverse and <4-µm axial resolution for OCM, and at 14-µm transverse and <3-µm axial resolution for OCT. Multiple sites on 75 tissue samples from 39 patients were imaged. OCM enabled cellular imaging of specimens from the upper and lower gastrointestinal tracts over a smaller field of view compared to OCT. Squamous cells and their nuclei, goblet cells in Barrett's esophagus, gastric pits and colonic crypts, and fine structures in adenocarcinomas were visualized. OCT provided complementary information through assessment of tissue architectural features over a larger field of view. OCM may provide a complementary imaging modality to standard OCT approaches for endoscopic microscopy.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/1.3322704
