Meioc maintains an extended meiotic prophase I in mice
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Soh-2017-Meioc maintains an extended meiotic p.pdf
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Author(s)
Soh, Ying Qi Shirleen
Mikedis, Maria M.
Kojima, Mina
Godfrey, Alexander Kamitsuka
Page, David C
de Rooij, Dirk G.
Date Issued
April 2017
Journal
PLoS Genetics
Publisher
Public Library of Science
Citation
Soh, Y. Q. Shirleen; Mikedis, Maria M.; Kojima, Mina; Godfrey, Alexander K.; de Rooij, Dirk G. and Page, David C. “Meioc Maintains an Extended Meiotic Prophase I in Mice.” Edited by Paula E. Cohen. PLOS Genetics 13, no. 4 (April 5, 2017): e1006704 © 2017 Soh et al
Version
Final published version
Abstract
The meiosis-specific chromosomal events of homolog pairing, synapsis, and recombination occur over an extended meiotic prophase I that is many times longer than prophase of mitosis. Here we show that, in mice, maintenance of an extended meiotic prophase I requires the gene Meioc, a germ-cell specific factor conserved in most metazoans. In mice, Meioc is expressed in male and female germ cells upon initiation of and throughout meiotic prophase I. Mouse germ cells lacking Meioc initiate meiosis: they undergo pre-meiotic DNA replication, they express proteins involved in synapsis and recombination, and a subset of cells progress as far as the zygotene stage of prophase I. However, cells in early meiotic prophase—as early as the preleptotene stage—proceed to condense their chromosomes and assemble a spindle, as if having progressed to metaphase. Meioc-deficient spermatocytes that have initiated synapsis mis-express CYCLIN A2, which is normally expressed in mitotic spermatogonia, suggesting a failure to properly transition to a meiotic cell cycle program. MEIOC interacts with YTHDC2, and the two proteins pull-down an overlapping set of mitosis-associated transcripts. We conclude that when the meiotic chromosomal program is initiated, Meioc is simultaneously induced so as to extend meiotic prophase. Specifically, MEIOC, together with YTHDC2, promotes a meiotic (as opposed to mitotic) cell cycle program via post-transcriptional control of their target transcripts.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1371/journal.pgen.1006704
