Generating single-domain antibodies against fibronectin splice variants
Name
986240630-MIT.pdf
Description
Full printable version
Size
5.63 MB
Format
Adobe PDF
Checksum (MD5)
206b039f6508ab10758795ad757daaef
Author(s)
Peterson, Alexis W
Advisor(s)
Richard Hynes.
Date Issued
2017
Publisher
Massachusetts Institute of Technology
Abstract
Here, I describe the process of generating single-domain antibodies which bind to splice variants of fibronectin containing EIIIA or EIIIB. Alpacas were immunized with either purified antigen cocktails, or from the ECM of tumor samples, and antibody libraries were generated. Using these libraries to pan against, I selected for VHH which bind to EIIIA and EIIIB. Since these splice variants are upregulated in tumor angiogenesis and are rarely seen elsewhere in adult tissues, antibodies targeting EIIIA or EIIIB may be of use for imaging tumors and metastases.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2017.
Cataloged from PDF version of thesis.
Includes bibliographical references (page 51).
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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