Understanding and Engineering Chromatin as a Dynamical System across Length and Timescales
Name
1-s2.0-S2405471220303689-main.pdf
Description
Published version
Size
13.61 MB
Format
Adobe PDF
Checksum (MD5)
407e471e0c551ba43d4fd7353ba521b4
Author(s)
Johnstone, Christopher P
Wang, Nathan B
Sevier, Stuart A
Galloway, Kate E
Date Issued
2020
Journal
Cell Systems
Publisher
Elsevier BV
Version
Final published version
Abstract
Connecting the molecular structure and function of chromatin across length and timescales remains a grand challenge to understanding and engineering cellular behaviors. Across five orders of magnitude, dynamic processes constantly reshape chromatin structures, driving spaciotemporal patterns of gene expression and cell fate. Through the interplay of structure and function, the genome operates as a highly dynamic feedback control system. Recent experimental techniques have provided increasingly detailed data that revise and augment the relatively static, hierarchical view of genomic architecture with an understanding of how dynamic processes drive organization. Here, we review how novel technologies from sequencing, imaging, and synthetic biology refine our understanding of chromatin structure and function and enable chromatin engineering. Finally, we discuss opportunities to use these tools to enhance understanding of the dynamic interrelationship of chromatin structure and function.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.cels.2020.09.011
