Enriched Protein Screening of Human Bone Marrow Mesenchymal Stromal Cell Secretions Reveals MFAP5 and PENK as Novel IL-10 Modulators
Name
Cima_Enriched protein.pdf
Size
657.34 KB
Format
Adobe PDF
Checksum (MD5)
ef23462a26258b3dd1a8388abaa404ad
Author(s)
Elman, Jessica S
Shen, Keyue
Gabow, Aaron
Yarmush, Joshua
Jiao, Yunxin
Fletcher, Anne
Lee, Jungwoo
Yarmush, Martin L
Parekkadan, Biju
Li, Matthew
Date Issued
December 2016
Journal
Molecular Therapy
Publisher
Elsevier BV
Citation
Milwid, Jack M et al. “Enriched Protein Screening of Human Bone Marrow Mesenchymal Stromal Cell Secretions Reveals MFAP5 and PENK as Novel IL-10 Modulators.” Molecular Therapy 22, 5 (May 2014): 999–1007 © The American Society of Gene & Cell Therapy
Version
Final published version
Abstract
The secreted proteins from a cell constitute a natural biologic library that can offer significant insight into human health and disease. Discovering new secreted proteins from cells is bounded by the limitations of traditional separation and detection tools to physically fractionate and analyze samples. Here, we present a new method to systematically identify bioactive cell-secreted proteins that circumvent traditional proteomic methods by first enriching for protein candidates by differential gene expression profiling. The bone marrow stromal cell secretome was analyzed using enriched gene expression datasets in combination with potency assay testing. Four proteins expressed by stromal cells with previously unknown anti-inflammatory properties were identified, two of which provided a significant survival benefit to mice challenged with lethal endotoxic shock. Greater than 85% of secreted factors were recaptured that were otherwise undetected by proteomic methods, and remarkable hit rates of 18% in vitro and 9% in vivo were achieved. © 2014 The American Society of Gene and Cell Therapy.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/MT.2014.17
